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mRNA alternative splicing in dog S1. (a) PCR amplification from exon 9 to the 3′ UTR of cDNAs from the four beagles experimentally infected with L. infantum. R1 and R2 are resistant dogs, and S1 and S2 are susceptible dogs. (b) PCR amplification of the same region of dog S1 from three different RNA extractions. Two independent RT-PCRs of each RNA extraction are shown (lanes 1 and 2). Lane M1, φX174 HaeIII; M2, 1-kb ladder.
