FIG. 6.

Quantitative measurement of the binding of wild-type, mutant, and recombinant H. ducreyi strains to plastic and HFF cells. Assays were performed as described in Materials and Methods. (A) Twenty-four-well plastic tissue culture plates; (B) ATCC CRL-7014 HFF monolayers. The experiments represented by bars 1 through 6 are a composite of two separate experiments. Bars: 1, wild-type 35000; 2, tadA mutant 35000.400; 3, rcpA mutant 35000.401; 4, wild-type 35000HP; 5, flp-1 flp-2 mutant 35000HP.402; 6, 35000HP.402(pJN031); 7, 35000HP.402(pJN032). Two additional experiments were performed with four strains, and the composite results are depicted in bars 8 through 11. Bars: 8, wild-type 35000HP; 9, flp-1 flp-2 mutant 35000HP.402; 10, flp-1 flp-2 cdtA mutant 35000HP.402.301; 11, 35000HP.402.301REP. The error bars indicate standard deviations.