TABLE 2.
Primers (5′-3′) used for RT-PCR and cloning procedures
| Primer | Sequence |
|---|---|
| p01 | AATTGCAGTCGCAGTTGC |
| p02 | ATAGCAGTAACACCTTTTTGG |
| p03 | AGTGGATTTTTATTTGCCC |
| p04 | CGAATATCGGTATAAGATAGCC |
| p05 | GAGGGTTGTTTTTCCGCC |
| p06 | GTGTATTTAGTTGGTCATCAGG |
| p07 | ACAAACGATGAAGCAACAG |
| p08 | GATTTCTGTTTTGCCTTCGCC |
| p09 | CACCGGTACGATGGAACG |
| p10 | GGTTTACGTGCGATTTCG |
| p11 | ACTGACTGAAGTGGCTGC |
| p12 | AAGCCGCGACTACGTAGC |
| p13 | ACAAGGTGGGCAATCTGG |
| p14 | GCTTACTCCGTTCATTTTCG |
| p15 | TTAACGCGTTCAGCCGTG |
| p16 | GAAGCCGATTAGTTCAGC |
| p17 | GGTGGTGCGATTATTCTCTAC |
| p18 | CCTTTCGGTTTTTCATCTTC |
| p19 | TCCGCATGTTCTGTACCG |
| p20 | CTAGTGGCTAGCGTTGTT |
| p21 | CGCCACAAAACAGTGAAGC |
| p22 | GAGCGGCTTCTGTAACTG |
| p23 | TCGCAGATAAAACACCAAC |
| p24 | TAGCCTGTAGAATAGCAACG |
| p25 | TCGCAGATAAAACACCAAC |
| p26 | TTTTCTGCGGTAAGGGAG |
| p31 | CGACATGCATGCTTAGCCGCAAAACAGACCGb |
| p32 | AACTGTGATCTTCAGGGTCG |
| p39 | AGAATAATGCGACCACCCG |
| p40 | GCCATAGTGACTCAATCTC |
| p41 | CACGATATCCGCCACACTATCCACCTATCCc |
| p42 | CATGCATGCTAATTATTAAAAGATACACTTb |
| p43 | CTCGTTTAGGCAGAGCGATTG |
| p44 | CATACGGGCTTCTGCTGTCA |
| p45a | TGCGAATAAAATGGAGCAAAAGAAGAAAGCA |
| p46 | CCTATTGTAGCCTTATTGTTTGTTTCA |
| p47 | GGCTTGCTCTAGTGCATCATTTAA |
| p48a | TAGAGGTAGCAGGCATAATTCAAGATAAGGCCAG |
| p49 | GCGGCAGGCTTAACACATG |
| p50 | TCCCCCTCCATAAGCCAGAT |
| p51a | CCTTGGGTGACGAGTGGCGGA |
a
Fluorescent probe labeled at the 5′ end with 6-carboxyfluorescein and at the 3′ end with Black Hole Quencher 1.
b
SphI site is underlined.
c
EcoRV site is underlined.