TABLE 3.
Lesion formation by wild-type and mutant strains of H. ducreyi in the temperature-dependent rabbit modela
| Strain | Inoculum size | Mean lesion score (SD)
|
P valueb | ||
|---|---|---|---|---|---|
| Day 2 | Day 4 | Day 7 | |||
| Expt A | |||||
| 35000 | 105 | 3.63 (0.52) | 4.00 (0) | 4.00 (0) | |
| 35000.400 | 105 | 3.63 (0.52) | 4.00 (0) | 4.00 (0) | 0.0156c |
| 35000 | 104 | 3.25 (0.46) | 3.38 (0.52) | 3.50 (0.53) | |
| 35000.400 | 104 | 3.00 (0) | 2.88 (0.35) | 2.38 (0.74) | |
| Expt B | |||||
| 35000 | 105 | 4.00 (0) | 4.00 (0) | 4.00 (0) | |
| 35000.400 | 105 | 3.75 (0.46) | 3.88 (0.35) | 3.88 (0.35) | 0.0125c |
| 35000 | 104 | 3.88 (0.35) | 3.88 (0.35) | 4.00 (0) | |
| 35000.400 | 104 | 3.25 (0.46) | 3.38 (0.52) | 3.38 (0.74) | |
| Expt C | |||||
| 35000HP | 105 | 4.00 (0) | 4.00 (0) | 4.00 (0) | |
| 35000HP.402 | 105 | 3.75 (0.46) | 3.88 (0.35) | 3.88 (0.35) | 0.2928d |
| 35000HP | 104 | 3.38 (0.52) | 3.00 (0.53) | 3.13 (0.35) | |
| 35000HP.402 | 104 | 3.38 (0.52) | 2.63 (0.91) | 2.75 (1.04) | |
| Expt D | |||||
| 35000HP | 105 | 3.75 (0.46) | 3.88 (0.35) | 3.88 (0.35) | |
| 35000HP.402 | 105 | 3.50 (0.53) | 3.63 (0.51) | 3.50 (0.53) | 0.9393d |
| 35000HP | 104 | 3.00 (0) | 3.00 (0.53) | 2.88 (0.64) | |
| 35000HP.402 | 104 | 3.50 (0.53) | 3.00 (1.07) | 3.00 (1.20) | |
a
Eight rabbits were used in each experiment.
b
P value calculated for the difference between wild-type and test strain lesion scores. P values were calculated by using the lesion scores from both inoculum sizes and from all 3 days. A P value of 0.0167 was needed for significance.
c
The tadA mutant was less virulent than the wild-type parent strain in both experiments.
d
The flp1 flp2 mutant was no less virulent than the wild-type parent strain in both experiments.