6.

CD73 inhibition partially reverses the immunosuppressive effect of adenosine generated from AMP in CD8 ⁺ T cells. CD8⁺ T cells were activated using CD3/CD28 activation beads in the presence of 2.5 μM EHNA, with or without 1 mM AMP and CD73 inhibitors (500 nM or 50 nM). A. CD25 expression. The percentage of CD8⁺ T cells expressing the activation marker CD25 was measured by flow cytometry 60 h after activation. Data represent technical triplicates. B. Cytokine secretion. Granzyme B and IFN-γ levels in the culture supernatant were quantified by ELISA 60 h after activation. The data represent technical duplicates from two independently treated wells. The figure shows representative data out of six replicates.