Table 6.

Intracellular p21 and cleaved caspase-3 levels (pg/µL) in BEAS-2B, A549, MDA-MB-231, and HCT116 cells following treatment with ME, HE, and cisplatin (5 µg/mL for 24 h)

	BEAS-2B*	A549*	MDA-MB-231*	HCT116*
	p21 Level (pg/µL)
Control	0,68 ± 0.11c	0,94 ± 0,07d	0,71 ± 0,05d	0,63 ± 0,04d
ME	3,33 ± 0.13a	2,52 ± 0,09b	3,76 ± 0,08b	2,73 ± 0,08b
HE	3,55 ± 0.09a	3,73 ± 0,09a	3,93 ± 0,03a	3,63 ± 0,13a
Cisplatin**	1,47 ± 0.10b	1,27 ± 0,08c	1,43 ± 0,06c	1,16 ± 0,05c
p Value	< 0.001	< 0.001	< 0.001	< 0.001
	Cleaved Caspase 3 Level (pg/µL)
Control	1,25 ± 0,06d	1,06 ± 0,09d	1,24 ± 0,08d	1,15 ± 0,07d
ME	7,45 ± 0,13b	4,69 ± 0,10b	9,02 ± 0,10b	4,95 ± 0,17b
HE	7,94 ± 0,02a	7,73 ± 0,07a	9,96 ± 0,14a	7,46 ± 0,29a
Cisplatin **	2,64 ± 0,08c	2,31 ± 0,10c	2,44 ± 0,09c	2,21 ± 0,07c
p Value	< 0.001	< 0.001	< 0.001	< 0.001

^*: Cell lines, ^**: Reference drug, ME: Acidified methanolic extract, HE: Hexane extract

Data are presented as mean ± standard deviation (SD) from three independent experiments. Different superscript letters (a-d) in the same column indicate statistically significant differences (p < 0.05)