TABLE 1.
C. pneumoniae proteins expressed in infected HEp-2 cells under normal conditions or IFN-γ-induced persistence
| Function | Designation | Mra | pIa | Effect of IFN-γb | Protein name |
|---|---|---|---|---|---|
| Outer membrane proteins | MOMP | 37.8 (36.0) | 6.8 (7.0) | + | Major outer membrane protein |
| Cell wall hydrolysis | AmiB | 25.2 (22.8) | 7.5 (8.7) | + | N-Acetylmuramoyl-l-Ala amidase |
| Chaperones | GroEL | 58.3 (58.2) | 5.5 (5.3) | + | Heat shock protein 60 |
| DnaK | 68.3 (71.3) | 5.1 (4.9) | NC | Heat shock protein 70 | |
| Glycolysis | PyK | 50.0 (54.6) | 6.5 (6.7) | NC | Pyruvate kinase |
| PgK | 41.9 (43.0) | 6.8 (6.1) | + | Phosphoglycerate kinase | |
| GlgP | 37.0 (94.5) | 6.1 (5.8) | + | Glycogen phosphorylase | |
| DNA replication | GyrA | 75.5 (93.8) | 5.7 (6.6) | + | DNA gyrase subunit A |
| Transcription | RpoA | 44.8 (41.8) | 5.2 (5.3) | + | DNA-directed RNA polymerase α chain |
| PnP | 79.8 (75.3) | 5.5 (5.4) | + | Polyribonucleotide nucleotidyltransferase | |
| Translation | ThrS | 70.2 (72.6) | 6.1 (5.8) | NC | Threonyl tRNA synthetase |
| PepA | 56.2 (54.7) | 6.2 (5.9) | NC | Leucine aminopeptidase | |
| GatA | 54.2 (53.6) | 5.1 (5.8) | NC | Glutamyl-tRNA amidotransferase A | |
| EF-TU | 44.0 (43.0) | 6.0 (5.4) | NC | Elongation factor Tu | |
| Rrf | 20.8 (20.1) | 8.0 (8.4) | + | Ribosome recycling factor | |
| Amino acid biosynthesis | Amn | 26.0 (32.6) | 7.5 (6.5) | + | AMP nucleosidase |
| Type III secretion | SctN | 52.1 (48.1) | 5.9 (5.8) | + | Type III secretion ATPase |
| Hypothetical proteins | 0512 | 56.1 (56.3) | 6.8 (6.2) | NC | Hypothetical protein CPN0512 |
| 0820 | 20.9 (21.6) | 5.7 (5.6) | NC | Hypothetical protein CPN0820 | |
| 0763 | 23.1 (26.7) | 5.9 (5.2) | NC | Hypothetical protein CPN0763 | |
| 0425 | 18.5 (21.3) | 4.6 (4.7) | NC | Hypothetical protein CPN0425 |
a
Theoretical values for Mr and pI of translated gene products are shown in parentheses and were obtained from the C. pneumoniae genome sequence database located in The Institute For Genomic Research server (http://www.tigr.org).
b
+, protein found to be significantly upregulated (P < 0.05) following IFN-γ treatment. NC, no significant change in expression.