TABLE 2.
Genes identified by INH selection in macrophages
| Category | Gene | Rv no.a | Comment |
|---|---|---|---|
| Fatty acid degradation | aceA | Rv0467 | Isocitrate lyase |
| fadA4 | Rv1323 | Acetyl-coenzyme A acetyltransferase | |
| echA19 | Rv3516 | Enoyl-coenzyme A hydratase | |
| fadA5 | Rv3546 | Acetyl-coenzyme A acetyltransferase | |
| Possible fatty acid metabolism | ephF | Rv0134 | Epoxide hydrolase |
| Rv0610c | Monooxygenase | ||
| Rv1144 | Alcohol dehydrogenase | ||
| Rv1774 | Oxidoreductase | ||
| Cell envelope | Rv0102 | Membrane | |
| Rv1171 | Hydrophobic protein | ||
| Rv2120c | Membrane | ||
| lppM | Rv2171 | Lipoprotein | |
| Rv3237c | Potassium channel | ||
| Rv3524 | Membrane, sensor | ||
| Rv3717 | N-Acetyl-muramoyl-L-alanine amidase | ||
| Intermediary metabolism | pckA | Rv0211 | Phosphoenol carboxykinase |
| eno | Rv1023 | Enolase | |
| PPE/PEPGRS | Rv0977 | PE/PGRS | |
| Rv1361c | PPE | ||
| Rv1840c | PE/PGRS | ||
| Putative transcriptional regulators | Rv0549c | Helix-turn-helix motif | |
| Rv2009 | Helix-turn-helix motif | ||
| Rv3321c | Helix-turn-helix motif | ||
| Miscellaneous | fusA2 | Rv0120c | Elongation factor G |
| proC | Rv0500 | Proline biosynthesis | |
| uvrC | Rv1420 | Exonuclease ABC | |
| infC | Rv1641 | Initiation factor 3 | |
| Rv2224c | Exported protease | ||
| nirA | Rv2391 | Nitrite reductase | |
| Rv2520c | Transmembrane domain | ||
| dut | Rv2697c | Deoxyuridine triphosphatase | |
| Rv3225c | Aminoglycoside 3′ phosphotransferase | ||
| Unknown | Rv0036c, Rv0406c, Rv0811c, Rv1778c, Rv2273, Rv2468c, Rv2632c, Rv2717c, Rv3427c, Rv3493c, Rv3717 |
a
The Rv number is the number assigned to the open reading frame, and the genes are annotated as described by the Pasteur Institute at the TUBERCULIST website (http://genolist.pasteur.fr/TubercuList).