TABLE 3.
M. tuberculosis genes induced during macrophage infection
| Gene | Induction ratiosa
|
||
|---|---|---|---|
| RPMI 1640 mediumb | 7H9 medium
|
||
| 24 h after infectionc | 72 h after infectionc | ||
| Induced genes | |||
| Rv2224c | 0.9 ± 0.1 | 4.0 ± 0.8 | 3.5 ± 0.7 |
| Rv3237c | 0.6 ± 0.0 | 3.6 ± 1.8 | 4.0 ± 1.2 |
| Rv3321c | 1.3 ± 0.2 | 2.3 ± 0.0 | 2.0 ± 0.2 |
| echA19 | 4.2 ± 0.4 | 25.0 ± 14.5 | 35.0 ± 13.5 |
| aceA | 9.5 ± 1.3 | 85.4 ± 3.1 | 88.4 ± 8.6 |
| fadA4 | 1.0 ± 0.3 | 2.6 ± 1.0 | 4.0 ± 1.5 |
| pckA | 2.2 ± 0.1 | 7.0 ± 2.0 | 6.1 ± 0.9 |
| Rv2520c | 1.1 ± 0.1 | 2.2 ± 1.3 | 0.9 ± 0.5 |
| Genes not induced | |||
| fadA5 | 1.5 ± 0.4 | 0.3 ± 0.1 | 0.5 ± 0.3 |
| Rv1171 | 1.0 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.1 |
| Rv0977 | 1.0 ± 0.0 | 0.5 ± 0.1 | 1.5 ± 0.3 |
| Rv1774 | 0.4 ± 0.1 | 0.1 ± 0.2 | 0.7 ± 0.0 |
| nirA | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.2 ± 0.1 |
a
The values are means ± standard errors based on triplicate samples.
b
The values are the relative amounts of M. tuberculosis mRNA obtained after 2 h of incubation in RPMI 1640 medium compared to the amounts of mRNA obtained after growth in broth. The values are normalized to sigA mRNA.
c
The values are the relative amounts of M. tuberculosis mRNA obtained 24 and 72 h after infection in THP-1 cells compared to the amounts of mRNA obtained after growth in broth. The values are normalized to sigA mRNA.