Fig. 5. Cellular experiments and immunohistochemistry. (A) Reverse transcription–polymerase chain reaction (RT-PCR) shows the knockdown efficiency of TMEM59. (B) Cell counting kit-8 assay indicates enhanced LNCaP and PC3 cells proliferation after knockdown. (C) Colony formation assay in si-TMEM59 and control cells (×10 objective lens and ×10 eyepiece). (D) TMEM59-knockdown promotes prostate cancer cell metastasis in LNCaP and PC3 cells (×20 objective lens and ×10 eyepiece). (E) Immunohistochemistry of TMEM59 in tumor and normal tissues (×4 objective lens, ×10 eyepiece, and ×20 objective,×10 eyepiece). ^*p<0.05, ^**p<0.01, ^***p<0.001.