TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Genotype	Relevant properties	Source or reference
S. aureus strains
RN4220		Restriction-deficient 8325-4 mutant	22
Newman		Expresses clumping factor	8
DU5944	Newman clfA2::Tn917 EmrclfB::Tcr	Deficient in clumping factors A and B	29
P1		Expresses FnBPs	37
P1 FnBP−	P1 fnbA::TcrfnbB::Emr	Deficient in FnBPA and -B	T. J. Foster
V329		Expresses Bap	7
m556	V329 bap::Tn917 Emr	Deficient in Bap	7
SA113		Expresses PIA/PNSG	6
SA113Δica	SA113 ΔicaADBC::Tcr	Deficient in PIA/PNSG	6
Plasmids
pBT2		Shuttle plasmid	3
pBT2:Bap		pBT2 with bap gene	7