Table 2.
PCR Primers
| Species | Gene symbols | Forward sequence | Reverse sequence |
|---|---|---|---|
| Human | SRD5A2 | 5′-GCAGTGTCTTAGTTGATGAG-3′ | 5′-TGTGGTTATTAAAACCTGGC-3′ |
| Human | WNT5A | 5′-GGCAAGTTGGTACAGGTCAA-3′ | 5′-ATTGCGCACGCAGTAGT-3′ |
| Human | CTNNB1 | 5′-CACAAGCAGAGTGCTGAAGGTG-3′ | 5′-GATTCCTGAGAGTCCAAAGACAG-3′ |
| Human | LEF1 | 5′-CTACCCATCCTCACTGTCAGTC-3′ | 5′-GGATGTTCCTGTTTGACCTGAGG-3′ |
| Human | TCF7L1 | 5′-TCAAGGACACGAGGTCACCATC-3′ | 5′-GGAGAAGTGGTCATTGCTGTAGG-3′ |
| Human | TCF7L2 | 5′-CCCTTTGCTCTTATATCTGACTTCT-3′ | 5′-TTCTGCACGCGTGAGTTT-3′ |
| Human | CCND1 | 5′-TCTACACCGACAACTCCATCCG-3′ | 5′-TCTGGCATTTTGGAGAGGAAGTG-3′ |
| Human | MYC | 5′-CCTGGTGCTCCATGAGGAGAC-3′ | 5′-CAGACTCTGACCTTTTGCCAGG-3′ |
| Human | CYCS | 5′-AAGGGAGGCAAGCACAAGACTG-3′ | 5′-CTCCATCAGTGTATCCTCTCCC-3′ |
| Human | CASP3 | 5′-GGAAGCGAATCAATGGACTCTGG-3′ | 5′-GCATCGACATCTGTACCAGACC-3′ |
| Human | CASP9 | 5′-GTTTGAGGACCTTCGACCAGCT-3′ | 5′-CAACGTACCAGGAGCCACTCTT-3′ |
| Human | PARP1 | 5′-TGATAGCAGCAAGGATCCCAT-3′ | 5′-CCGTGCCACAGCAATCTTCG-3′ |
| Human | CACNA2D3 | 5′-GAACATCCCGATGTGTCCTTGG-3′ | 5′-ACTGGAGCAGAGGTTCTTTGCC-3′ |
| Human | CAMKIIA | 5′-GAGCCATTCTCACCACGATGCT-3′ | 5′-TGGTGTTGGTGCTCTCTGAGGA-3′ |
| Human | PTPRK | 5′-GGACTGAATCCTGGCACTTT-3′ | 5′-GCCAATCTCTACCCGTGAATC-3′ |
| Human | RYK | 5′-CAGCAAGACCTGGTACACATGG-3′ | 5′-CAAGTCTCTGGAGAGGGCATTG-3′ |
| Human | ROR1 | 5′-GAGGCAACCAAAACACGTCAGAG-3′ | 5′-GGCACACTCACCCAATTCTTCC-3′ |
| Human | ROR2 | 5′-GTACGCATGGAACTGTGTGACG-3′ | 5′-AAAGGCAAGCGATGACCAGTGG-3′ |
| Human | LRP6 | 5′-CAGTTGGAGTGGTGCTGAAAGG-3′ | 5′-CCATCCAAAGCAGCCCGTTCAA-3′ |
| Human | TGFBR1 | 5′-TCGAGATAGGCCGTTTGTATG-3′ | 5′-GCAATGGTCCTGATTGCAG-3′ |
| Human | IGF1 | 5′-CTCTTCAGTTCGTGTGTGGAGAC-3′ | 5′-CAGCCTCCTTAGATCACAGCTC-3′ |
| Human | IGF2 | 5′-TGGCATCGTTGAGGAGTGCTGT-3′ | 5′-ACGGGGTATCTGGGGAAGTTGT-3′ |
| Human | VEGFA | 5′-CGAGGTTCACCCTCAGTTTC-3′ | 5′-GCACGACTCCTTCTCCAAATA-3′ |
| Human | VEGFB | 5′-AAGGACAGTGCTGTGAAGCCAG-3′ | 5′-TGGAGTGGGATGGGTGATGTCA-3′ |
| Human | TGFB1 | 5′-TACCTGAACCCGTGTTGCTCTC-3′ | 5′-GTTGCTGAGGTATCGCCAGGAA-3′ |
| Human | TGFB2 | 5′-GATGACGACGACAACGATGA-3′ | 5′-GTCTGAACTAGTACCGCCTTT-3′ |
| Human | TGFB3 | 5′-CTAAGCGGAATGAGCAGAGGATC-3′ | 5′-TCTCAACAGCCACTCACGCACA-3′ |
| Human | GAPDH | 5′-ACAGTTGCCATGTAGACC-3′ | 5′-TTGAGCACAGGGTACTTTA-3′ |
| Mouse | Srd5a2 | 5′-CGGTGTCTTCTTCTTTATTCTG-3′ | 5′-ATCCATTCAATAATCTCGCC-3′ |
| Mouse | Wnt5a | 5′-ATCTTGGGCACAGGGATTG-3′ | 5′-CAAATGTGGGCGTGATTGTG-3′ |
| Mouse | Lef1 | 5′-ACTGTCAGGCGACACTTCCATG-3′ | 5′-GTGCTCCTGTTTGACCTGAGGT-3′ |
| Mouse | Tcf7l1 | 5′-GACCTCGGAGCGAACGA-3′ | 5′-TTGACCAGGGACGACTTGA-3′ |
| Mouse | Xrcc4 | 5′-GACATGGCTATGGAGAAAGGAA-3′ | 5′-GAGAAATGCCGAGACTCCTTAG-3′ |
| Mouse | Zfp143 | 5′-GCGCCCATCACCCTATTT-3′ | 5′-ACGAGTGAATTATGCCGATTCT-3′ |
| Mouse | Zmiz1 | 5′-GCAGAGCAAGCGAGCAG-3′ | 5′-GCGAGAGCGCGGAAATC-3′ |
| Mouse | Ptprk | 5′-CTGCCTACCATGAAGGAGAATG-3′ | 5′-GGATATTCAAAGTGCCAGGGT-3′ |
| Mouse | Ryk | 5′-ACGACCCAGTATCTGAGAGCTG-3′ | 5′-GGCTTTGGCTTCCAGAAGAGTG-3′ |
| Mouse | Ror2 | 5′-AACGTACGCGTGGAACTGTGTG-3′ | 5′-CAGACGAGGAAGAACAGGCAAG-3′ |
| Mouse | Lrp6 | 5′-CTGAATGCTGACAACAGGACCTG-3′ | 5′-GACGTTCCGAAGGCTGTGGATA-3′ |
| Mouse | Tgfb1 | 5′-CCTGTGAGACAGATGGTCTTTG-3′ | 5′-TGGCCTGTCTCGAGGAATTA-3′ |
| Mouse | Tgfb2 | 5′-CAGATCCTGAGCAAGCTGAA-3′ | 5′-GTCCCTGGTACTGTTGTAGATG-3′ |
| Mouse | Vegfa | 5′-CGGGATTGCACGGAAACT-3′ | 5′-CAGCTCCGATCGGTTTGTC-3′ |
| Mouse | Gapdh | 5′-GACAACTTTGGCATTGTGGAAG-3′ | 5′-TGCTTCACCACCTTCTTGATGT-3′ |