FIG. 2.

Dose-dependent effect of LPS on the expression of ABCA1 (A), SR-B1 (B), and IL-1β (C) genes and SR-B1 protein (D). Cultured cells were exposed to the increasing concentrations (0, 0.2, 2, 20, and 200 ng/ml) of LPS for 24 h. Levels of mRNA expression for the indicated genes were tested by RT-PCR analyses, and SR-B1 protein expression was estimated by Western blot analysis. For further steps, see the Fig. 1 legend. The results shown represent one of two experiments that yielded similar results. IOD, integrated optical density. Error bars indicate standard deviations.