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Effect of protease inhibitor TPCK and its structural analogue TLCK on LPS-induced changes in IL-1β, SR-B1, and ABCA1 mRNA expression. The cells were incubated with TPCK or TLCK (negative control) alone for 2 h prior to LPS addition and then for 22 h in the presence of LPS (10 ng/ml). After the incubation, total mRNA was isolated and the samples were analyzed by RT-PCR. The results shown represent one of two separate experiments that yielded similar results.
