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TLC fractionation of the acyl-HSL activity produced in RPMI. Normal-phase TLC of the acyl-HSLs present in cell-free supernatant of B. melitensis 16M Nalr (ER16M) was revealed by overlay with E. coli (pSB1075). Samples were spotted on a silica plate and developed with 45% acetone in N-hexane. Lane A, positive control (5 ng of C8-HSL); lanes B to D, fractions 4 to 6 of ER16M, respectively; lane E, negative control (ER); lane F, ER16M.
