TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| B. melitensis 16M Nalr | Spontaneous nalidixic acid-resistant mutant of the reference strain B. melitensis 16 M (Brucella Culture Collection of Nouzilly) | 62 |
| B. suis 1330 | B. suis biovar 1; reference strain (Brucella Culture Collection of Nouzilly) | ATCC 23444 |
| C. violaceum CV026 | Mini-Tn5 mutant derived from C. violaceum ATCC 31532 Hgr, cvil::Tn5xylE, Kmr, plus spontaneous Smr, acyl-HSL biosensor producing a purple pigment | 31 |
| E. coli JM109 | F′ traD36, proAB, laclq, lacZΔM15/recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac-proAB), mcrA | Promega |
| E. coli S17/1 λpir | λ-pir lysogen of S17-1 (thi pro hsdR−hsdM+recA RP4 2-Tc::Mu-Km::Tn7(Tpr Smr) | 52, 57 |
| Plasmids | ||
| pSB401 | luxR luxl′ (Photobacterium fischeri [ATCC 7744])::luxCDABE (Photorhabdus luminescens [ATCC 29999]) fusion; pACYC184-derived, Tcr, acyl-HSL biosensor producing bioluminescence | 68 |
| pSB1075 | lasR lasl′ (P. aeruginosa PAO1)::luxCDABE (P. luminescens [ATCC 29999]) fusion in pUC18 Apr, acyl-HSL biosensor producing bioluminescence | 68 |