FIG. 4.

Suppression of IL-2 production is mediated by a soluble factor. Purified OVA-TCR Tg CD4 T cells were placed with spleen cells from uninfected mice in the inner well of a transwell culture system. Cells from the following sources were added to the outer well; spleen cells from uninfected mice (Un-Spl), spleen cells from infected mice (Inf-Spl), CD11b⁺ cells from infected mice (Inf-11b), or spleen cells depleted of CD11b⁺ cells (Inf-11b Depl). Cultures were stimulated with 1 mg of OVA per ml. Supernatants were collected for IL-2 measurement 24 h later and measured in triplicate. Results from two separate experiments are shown, and data are expressed as means ± standard deviations. ∗, values significantly different from that for Un-Spl; #, value significantly different from that for Inf-Spl (P < 0.05 by one-way ANOVA).