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The suppressive factor is not TGF-β. APC (purified CD11b+ cells) from uninfected or infected B10.D2 mice were cultured with TCR Tg cells and OVA in the presence of anti-TGF-β or an isotype-matched control antibody at the indicated concentrations. The antibody was added at the beginning of the culture period. Supernatants were analyzed for IL-2 or IFN-γ 24 h later. Error bars indicate standard deviations.
