TABLE 2.
T-cell hybridomas produce IL-2 when antigen is presented on fixed APC from infected mice
| Expt no. | Stimulus | IL-2 (mean pg/ml ± SD) with the following source of APCa:
|
|||
|---|---|---|---|---|---|
| Uninfected spleen | Infected spleen | Uninfected spleen, fixed | Infected spleen, fixed | ||
| 1 | OVA | 159.7 | 4.2 | 381.7 | 330.7 |
| None | NDb | ND | 6.8 | 6.8 | |
| 2 | OVA | 229 ± 80 | 37 ± 5 | 423.2 ± 22 | 354.5 ± 36 |
| None | 31.9 ± 5 | ND | 3.8 ± 0.4 | 2.4 ± 0.3 | |
a
IL-2 in tissue culture supernatants of DO11.10 T cell hybridomas cultured with indicated APC. In experiment 1, only duplicate wells were cultured, so no standard deviation is given. The DO11.10 hybridoma is derived from a BALB/c mouse; therefore BALB/c APC were used.
b
ND, not done.