TABLE 3.
Effect of NaCl and human serum on antibacterial activity of peptides
| Bacterium | % Bacterial killing in presence of NaCl (in presence of human serum)a
|
|||
|---|---|---|---|---|
| HNP-1 | HNP-1ΔC | HNP-1ΔC18 | HNP-1ΔC18A | |
| E. coli W 160-37 | 68 (50) | 81 (47) | 68 (100) | 59 (100) |
| P. aeruginosa NCTC 6750 | 45 (0) | 10 (13) | 12 (87) | 41 (98) |
| S. aureus NCTC 8530 | 33 (0) | 92 (24) | 63 (99) | 84 (99) |
a
Mid-log-phase bacteria (105 CFU/ml) were incubated with the LCs of peptides in the presence of 100 mM NaCl. The values in parentheses correspond to the effect of human serum on antibacterial activity in the absence of NaCl. Peptides were used at their LCs and were added after 15 minutes of incubation with 1 mg/ml of serum. The values reported are the averages of three independent experiments. The variation was within ±5%.