TABLE 2.
β-Galactosidase activities of ampC and ampR promoters
| Strain | Relevant propertiesa | β-Galactosidase activityb
|
|
|---|---|---|---|
| Noninduced | Inducedc | ||
| PKM302 | PAO1, PampR-lacZ | 77.1 ± 8.7 | 123.0 ± 1.2 |
| PKM304 | PAOampR, PampR-lacZ | 96.3 ± 15.2 | 106.0 ± 16.0 |
| PKM301 | PAO1, PampC-lacZ | 124.1 ± 11.6 | 1,644.2 ± 33.7d |
| PKM303 | PAOampR, PampC-lacZ | 113.2 ± 7.5 | 122.3 ± 7.4e |
| PKM305 | PAO1, promoterless lacZ | ND | ND |
| PKM306 | PAOampR, promoterless lacZ | ND | ND |
a
The lacZ transcriptional reporter fusions were integrated into the attB site in the respective strains.
b
β-Galactosidase activities were expressed in Miller units. ND, nondetectable.
c
Induction was carried out using 500 μg/ml of benzylpenicillin.
d
P value of <0.05 compared between noninduced and induced conditions within the strain.
e
P value of <0.05 compared between PAO1 and PAOampR under the same condition.