TABLE 5.
Transcriptional activity of lasA, lasB, lasI, lasR, rhlI, and rhlR promoters
| Promoter fusiona | β-Galactosidase (Miller units)
|
|||
|---|---|---|---|---|
| Noninduced
|
Inducedb
|
|||
| PAO1 | PAOampR | PAO1 | PAOampR | |
| lasA | 790.0 ± 128.9 | 1,970.5 ± 312.6c | 1,364.7 ± 67.0d | 2,842.0 ± 286.4d |
| lasB | 709.3 ± 43.7 | 676.0 ± 75.1 | 773.0 ± 112.0 | 650.5 ± 67.3 |
| lasR | 2081.3 ± 63.2 | 6,904.0 ± 256.5c | 783.4 ± 136.9d | 6,805.8 ± 173.6c |
| lasI | 7,488.5 ± 193.8 | 12,985.3 ± 209.7c | 8,168.7 ± 405.2 | 12,413.7 ± 179.1c |
| rhlR | 9,188.8 ± 475.6 | 6,259.5 ± 26.1c | 10,375.5 ± 563.6 | 8,711.5 ± 301.6c |
| rhlI | 7,880.9 ± 784.1 | 9,624.4 ± 509.7 | 7,316.2 ± 796.4 | 9,245.8 ± 550.2 |
| Vector | 353.0 ± 33.7 | 316.7 ± 37.0 | 327.0 ± 24.8 | 327.7 ± 20.5 |
a
The promoter-lacZ fusions are pLP170 derivatives as described in Table 1.
b
Induction was carried out using 500 μg/ml of benzylpenicillin.
c
P value of <0.05 compared between PAO1 and PAOampR under the same condition.
d
P value of <0.05 compared between noninduced and induced conditions within the same strain.