TABLE 2.
MBL-producing Pseudomonas spp. and Acinetobacter spp. detected at a tertiary-care hospital in 2003-2004
| Species (no. of isolates) | No. of imipenem- resistant isolates | No. (%) of isolates positive for:
|
|||
|---|---|---|---|---|---|
| MBL screeninga | blaIMP-1 | blaVIM-2 | blaSIM-1 | ||
| P. aeruginosa (5,970) | 644 | 34 (5) | 2 (6) | 32 (94) | 0 |
| P. putida/P. fluorescens (211) | 45b | 42 (93) | 0 | 42 (100) | 0 |
| Acinetobacter spp. (3,294) | 545 | 135 (25) | 45 (33) | 83 (61) | 7 (6) |
| Total (9,475) | 1,234 | 211 (17) | 47 (22) | 157 (74) | 7 (4) |
a
By the Hodge test and the imipenem and EDTA-SMA double-disk synergy test.
b
Phenotypic differentiation of P. fluorescens and P. putida was performed for all imipenem-resistant isolates. The phenotypic identification of P. putida was confirmed by sequencing 16S rRNA from four randomly selected isolates.