Pulsed-field gel electrophoresis of the 20 E. coli strains after SpeI digestion. Eleven distinct clones were identified. λ lane, molecular size markers of lambda ladder. Lanes 1 to 6, clone A. Lanes 7 to 8, clone C. Lanes 9 and 10, clone I. Lanes 11 and 12, clone D. Lanes 13 and 14, clone K. Lanes 15 to 20, clones F, G, H, J, B, and E, respectively.