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. 2005 Nov;49(11):4745–4750. doi: 10.1128/AAC.49.11.4745-4750.2005

FIG. 2.

FIG. 2.

Electrophoresis of plasmid DNA of strains belonging to clones A, C, I, D and K (panel A), and Southern blot hybridization of the plasmid DNAs using CTX-M-2 probe (panel B). Panel A, plasmid pAFF2 (85 to 90 kb), used as a molecular size marker. Lanes 1 to 6, plasmid DNA of six strains belonging to PFGE type-A (strains 1020, 1438, 1241, 1254, 1131, and 1482, respectively). Lanes 7 and 8, 9 and 10, 11 and 12, and 13 and 14, plasmid DNA from pairs of strains belonging to the same PFGE type, strains 1031 and 1227 (clone C); strains 1299 and 1313 (clone I); strains 1266 and 1292 (clone D); and strains 1356 and 1326 (clone K), respectively. The arrows indicate plasmids that are common within the same clone. Panel B, Southern blot analysis of the plasmid DNAs digested with ApaI restriction enzyme, using blaCTX-M-2 as a probe. DNA size standards (kb) are shown on the right.