TABLE 1.
Primers used for PCR amplification for the detection of bla genes
| bla genea | Sequenceb | Product length (bp) | Reference |
|---|---|---|---|
| TEM Full | F: KACAATAACCCTGRTAAATGC | 936 | 28 |
| R: AGTATATATGAGTAAACTTGG | |||
| SHV Full | F: TTTATCGGCCYTCACTCAAGG | 930 | 28 |
| R: GCTGCGGGCCGGATAACG | |||
| OXA-2 Full | F: ATGGCAATCCGAATCTTCG | 828 | This study |
| R: TTATCGCGCAGCGTCCGAG | |||
| CTX-M-2 | F: ATGATGACTCAGAGCATTCG | 884 | 3, 31 |
| R: TTATTGCATCAGAAACCGTG | |||
| CTX-M-deg | F: CGYTTTSCIATGTGCAG | 550 | 28 |
| R: ACCGCRATATCRTTGGT | |||
| CTX-M-10 | F: GCAGCACCAGTAAAGTGATGG | 524 | 25, 31 |
| R: GCGATATCGTTGGTGGTACC | |||
| FEC-1 | F: GCGATAACGTGGCGATGAATAAGC | 407 | This study |
| R: GTTGAGGCTGGGTGAAGTAAGTGA | |||
| CTX-M-25 | F: CACACGAATTGAATGTTCAG | 924 | This study |
| R: TCACTCCACATGGTGAGT | |||
| CTX-M-8 | F: ATGATGAGACATCGCGTTAAG | 864 | This study |
| R: CGGTGACGATTTTCGCGGCAG | |||
| OXA-1 | F: ACACAATACATATCAACTTCGC | 813 | 27, 31 |
| R: AGTGTGTTTAGAATGGTGATC | |||
| OXA-10 | F: CGTGCTTTGTAAAAGTAGCAG | 651 | 31 |
| R: CATGATTTTGGTGGGAATGG | |||
| PER-1 | F: ATGAATGTCATTATAAAAGC | 925 | 24 |
| R: AATTTGGGCTTAGGGCAGAA |
a
CTX-M-deg, degenerate primers.
b
K is G or T; R is A or G; Y is C or T; S is G or C; I is inosine.