TABLE 2.
Coding regions of pAPEC-O2-R
| Coding sequence | Coordinates | Function of closest protein match | Source | % Identity | GenBank accession no. |
|---|---|---|---|---|---|
| yacC | 865-17 | Exonuclease | Escherichia coli plasmid ColIb-P9 | 98 | BAA75091 |
| yacB | 1192-911 | Unknown | Escherichia coli plasmid ColIb-P9 | 98 | BAA75090 |
| yacA | 1458-1189 | Unknown | Escherichia coli plasmid ColIb-P9 | 97 | BAA75089 |
| repA4 | 2095-1646 | Stable inheritance | Escherichia coli plasmid R100 | 100 | NP_052991 |
| repA1 | 3271-2340 | Plasmid replication | Escherichia coli plasmid B171 | 98 | NP_053107 |
| repA3 | 3448-3221 | Plasmid replication | Escherichia coli plasmid TUC100 | 79 | AAM14716 |
| repA2 | 3788-3531 | Negative regulator of plasmid replication | Escherichia coli plasmid R100 | 63 | NP_052988 |
| yihA | 4618-4028 | Unknown | Escherichia coli plasmid C15-1a | 99 | AAR25121 |
| hha | 4865-4656 | Modulating protein | Escherichia coli plasmid R100 | 100 | YP_053130 |
| yigB | 5465-4911 | Unknown | Escherichia coli plasmid C15-1a | 100 | AAR25120 |
| orf11 | 5813-5571 | Conserved hypothetical protein | Escherichia coli plasmid 1658/97 | 57 | AAO49551 |
| finO | 6563-5958 | Fertility inhibition protein | Escherichia coli plasmid R100 | 100 | BAA78888 |
| yieA | 7481-6621 | Unknown | Escherichia coli plasmid C15-1a | 100 | AAR25115 |
| traX | 8292-7540 | F pilus acetylation | Escherichia coli plasmid R100 | 97 | BAA78886 |
| traI | 13582-8306 | DNA helicase | Escherichia coli plasmid R100 | 97 | NP_052981 |
| traD | 15726-13576 | Coupling | Escherichia coli plasmid R100 | 97 | NP_052980 |
| traT | 16803-16027 | Surface exclusion and serum resistance | Escherichia coli plasmid F | 99 | BAA97971 |
| traS | 17320-16790 | Entry exclusion | Escherichia coli plasmid F | 79 | BAA78881 |
| traG | 20130-17308 | Pilus assembly | Escherichia coli plasmid R100 | 93 | NP_052976 |
| traH | 21503-20127 | Pilus assembly | Escherichia coli plasmid F | 99 | BAA97968 |
| trbJ | 21802-21500 | Plasmid transfer | Escherichia coli plasmid R100 | 86 | NP_052973 |
| trbB | 22337-21792 | Unknown | Escherichia coli plasmid F | 97 | BAA97965 |
| traQ | 22608-22324 | Pilus biosynthesis | Escherichia coli plasmid F | 98 | BAA97964 |
| trbA | 23074-22727 | Unknown | Escherichia coli plasmid F | 89 | BAA97962 |
| traF | 23833-23090 | Unknown | Escherichia coli plasmid F | 99 | BAA97961 |
| trbE | 24083-23826 | Unknown | Escherichia coli plasmid F | 94 | BAA97960 |
| traN | 25918-24110 | Mating pair stabilization | Escherichia coli plasmid F | 99 | BAA97959 |
| trbC | 26517-25915 | Pilus assembly | Escherichia coli plasmid F | 99 | BAA97958 |
| traU | 27554-26562 | Pilus assembly | Escherichia coli plasmid R100 | 99 | NP_052963 |
| traW | 28138-27551 | Pilus assembly | Escherichia coli plasmid F | 99 | BAA97956 |
| trbI | 28566-28180 | Plasmid transfer | Escherichia coli plasmid R100 | 99 | NP_052961 |
| traC | 31193-28563 | Pilus assembly | Escherichia coli plasmid F | 99 | BAA97956 |
| yfiC | 31681-31319 | Unknown | Escherichia coli plasmid R100 | 79 | NP_052959 |
| orf34 | 31924-31709 | Conserved hypothetical protein | Escherichia coli plasmid R100 | 96 | NP_052958 |
| yfiA | 32477-32004 | Unknown | Escherichia coli plasmid R100 | 94 | NP_052957 |
| traV | 32761-32276 | Plasmid transfer | Escherichia coli plasmid 1658/97 | 100 | AAO49525 |
| trbG | 33524-33273 | Plasmid transfer | Escherichia coli plasmid F | 98 | BAA97951 |
| trbD | 33828-33521 | Plasmid transfer | Escherichia coli plasmid F | 89 | NP_061459 |
| traP | 34407-33835 | Pilus expression | Escherichia coli plasmid ColB2 | 97 | AAB07776 |
| traB | 35824-34397 | Pilus assembly | Escherichia coli plasmid F | 100 | BAA97948 |
| traK | 36552-35824 | Pilus assembly | Escherichia coli plasmid F | 100 | BAA97947 |
| traE | 37105-36539 | Pilus assembly | Escherichia coli plasmid F | 99 | BAA97946 |
| traL | 37438-37127 | Pilus assembly | Escherichia coli plasmid F | 100 | BAA97945 |
| traA | 37812-37453 | Plasmid transfer | Escherichia coli plasmid 1658/97 | 97 | AAO49517 |
| traY | 38166-37846 | Plasmid transfer | Escherichia coli plasmid ColB4 | 97 | AAB04665 |
| traJ | 38853-38167 | Plasmid transfer regulation | Escherichia coli plasmid R1 | 98 | P05837 |
| traM | 39427-39044 | Plasmid transfer | Escherichia coli plasmid ColB4-K98 | 98 | P18807 |
| ygfA | 40351-39758 | Unknown | Escherichia coli plasmid F | 97 | BAA97940 |
| ygeB | 41469-40648 | Unknown | Escherichia coli plasmid F | 99 | BAA97939 |
| orf50 | 41650-41922 | Hypothetical protein | |||
| orf51 | 42108-41902 | Hypothetical protein | |||
| orf52 | 42356-42144 | Hypothetical protein | |||
| orf53 | 42279-42512 | Hypothetical protein | |||
| hok | 42956-42798 | Postsegregation killing | Escherichia coli plasmid R100 | 100 | NP_052939 |
| sok | 42988-43224 | Postsegregation killing | Escherichia coli plasmid R1 | 100 | P13971 |
| psiA | 43955-43236 | SOS inhibition | Escherichia coli plasmid F | 100 | NP_061443 |
| psiB | 44437-43952 | SOS inhibition | Escherichia coli plasmid F | 99 | SO1898 |
| orf58 | 46399-44441 | Conserved hypothetical protein | Escherichia coli plasmid F | 93 | BAA75128 |
| ykfF | 46748-46464 | Unknown | Escherichia coli plasmid F | 97 | AAD47188 |
| ssb | 47298-46759 | Single-stranded DNA binding | Escherichia coli plasmid F | 98 | BAA97930 |
| orf61 | 47530-47324 | Hypothetical protein | |||
| orf62 | 47984-47532 | Hypothetical protein | |||
| orf63 | 48148-47985 | Hypothetical protein | |||
| ydcA | 48712-48149 | Unknown | Escherichia coli plasmid R100 | 97 | NP_052920 |
| ydbA | 50121-48760 | Unknown | Escherichia coli plasmid R100 | 99 | NP_052919 |
| ydaB | 50403-50173 | Unknown | Escherichia coli plasmid R100 | 100 | NP_052918 |
| orf67 | 50667-51089 | Conserved hypothetical protein | Escherichia coli plasmid 1658/97 | 82 | AAO49640 |
| orf68 | 51632-51441 | Hypothetical protein | |||
| yfhA | 52051-51629 | Unknown | Escherichia coli plasmid F | 96 | BAA97928 |
| yciB | 52536-52098 | Antirestriction protein | Escherichia coli plasmid C15-1a | 99 | NP_957575 |
| orf71 | 52654-52824 | Hypothetical protein | |||
| ychA | 53711-52935 | Unknown | Escherichia coli plasmid R100 | 97 | NP_052912 |
| orf73 | 54206-53757 | Unknown | Escherichia coli plasmid O157 | 93 | AAC70143 |
| orf74 | 54426-54205 | Hypothetical protein | |||
| yfeA | 55110-54427 | DNA methylase | Escherichia coli plasmid F | 94 | BAA97922 |
| orf76 | 55530-55186 | Conserved hypothetical protein | Shigella flexneri plasmid WR100 | 97 | CAC05844 |
| yfdA | 55931-55494 | d-Serine permease | Escherichia coli plasmid F | 94 | BAA97920 |
| yfcB | 56419-55913 | Glutamine methyltransferase | Escherichia coli plasmid F | 94 | BAA97919 |
| impC | 56813-57061 | UV protection | Salmonella enterica plasmid SC137 | 100 | AAS76415 |
| impA | 57058-57495 | UV protection | Salmonella enterica plasmid SC137 | 100 | AAS76416 |
| impB | 57495-58766 | UV protection | Shigella flexneri SA100 virulence plasmid | 100 | AAD03593 |
| stbB | 59163-58771 | Stable plasmid inheritance | Escherichia coli plasmid B171 | 95 | NP_053129 |
| stbA | 60139-59168 | Stable plasmid inheritance | Escherichia coli plasmid B171 | 100 | NP_053130 |
| parA | 60368-61012 | Plasmid partitioning | Escherichia coli plasmid B171 | 99 | BAA84904 |
| orf85 | 61006-61281 | Conserved hypothetical protein | Escherichia coli plasmid B171 | 100 | NP_053132 |
| rsvB | 62201-61419 | Resolvase | Escherichia coli plasmid B171 | 88 | NP_053133 |
| orf87 | 62877-62269 | Hypothetical protein | |||
| orf88 | 63439-63035 | Hypothetical protein | |||
| orf89 | 64850-63876 | Conserved hypothetical protein | Klebsiella pneumoniae plasmid LVPK | 97 | NP_943494 |
| hnh | 65488-66396 | Endonuclease | Klebsiella pneumoniae plasmid LVPK | 99 | NP_943492 |
| orf91 | 67185-66784 | Conserved hypothetical protein | Klebsiella pneumoniae plasmid LVPK | 99 | NP_943490 |
| silE | 67772-67278 | Silver and heavy metal resistance | Klebsiella pneumoniae plasmid LVPK | 98 | NP_943489 |
| silS | 69435-67960 | Silver and heavy metal resistance | Klebsiella pneumoniae plasmid LVPK | 100 | NP_943488 |
| silR | 70108-69428 | Silver and heavy metal resistance | Klebsiella pneumoniae plasmid LVPK | 98 | NP_943487 |
| silC | 70298-71683 | Silver and heavy metal resistance | Klebsiella pneumoniae plasmid LVPK | 97 | NP_943486 |
| orf96 | 71711-72064 | Silver and heavy metal resistance | Klebsiella pneumoniae plasmid LVPK | 100 | NP_941215 |
| silB | 72178-73470 | Silver and heavy metal resistance | Klebsiella pneumoniae plasmid LVPK | 98 | NP_943483 |
| silA | 73481-76627 | Silver and heavy metal resistance | Klebsiella pneumoniae plasmid LVPK | 98 | NP_943482 |
| orf99 | 76714-77154 | Conserved hypothetical protein | Klebsiella pneumoniae plasmid LVPK | 98 | NP_943481 |
| silP | 77268-79729 | Silver and heavy metal resistance | Klebsiella pneumoniae plasmid LVPK | 98 | NP_943480 |
| orf101 | 80711-80001 | Conserved hypothetical protein | Klebsiella pneumoniae plasmid LVPK | 99 | NP_943478 |
| tnpA | 80762-81466 | IS26 transposase | Escherichia coli | 100 | CAD43299 |
| pecM | 81472-81867 | Unknown | Escherichia coli plasmid C15-1a | 94 | NP_957550 |
| tetA | 83098-81899 | Tetracycline resistance | Escherichia coli | 99 | AAT37966 |
| tetR | 83177-82858 | Tetracycline repressor | Escherichia coli | 100 | AAT37964 |
| orf106 | 84128-83886 | Relaxase and helicase | Salmonella enterica plasmid SC138 | 98 | AAS76290 |
| tnpA | 87152-84156 | IS1721 transposase | Escherichia coli | 99 | JQ1477 |
| tnpR | 87716-87156 | IS1721 resolvase | Escherichia coli | 99 | CAA46340 |
| tnpM | 88476-87892 | Tn21 modulator | Escherichia coli | 100 | AAC33910 |
| intI1 | 89458-88445 | Integrase | Escherichia coli plasmid R100 | 100 | NP_052898 |
| folA | 89604-90088 | Trimethoprim resistance | Escherichia coli plasmid R721 | 99 | NP_065309 |
| catB3 | 90206-90838 | Chloramphenicol resistance | Escherichia coli plasmid HSH2 | 100 | AAP20921 |
| aadA5 | 90896-91684 | Streptomycin and spectinomycin resistance | Escherichia coli | 100 | AAV69850 |
| qacEΔ1 | 91893-92237 | Quaternary ammonium resistance | Escherichia coli plasmid 1658/97 | 100 | AAO49596 |
| sulI | 92231-93070 | Sulfonamide resistance | Escherichia coli plasmid R100 | 99 | NP_052895 |
| orf116 | 93198-93698 | Conserved hypothetical protein | Escherichia coli plasmid 1658/97 | 100 | AAO49594 |
| istB | 94656-93874 | Tn21 transposition | Shigella flexneri Tn21 | 100 | AAC33916 |
| istA | 96160-94646 | IS1326 transposase | Klebsiella pneumoniae plasmid RMH760 | 99 | AAM89412 |
| tniBΔ1 | 96468-96271 | Transposon ATPase | Escherichia coli plasmid R100 | 99 | NP_052890 |
| tnpA | 99466-96461 | Tn3 transposase | Escherichia coli | 99 | P03008 |
| orf121 | 99628-100185 | Tn3 resolvase | Escherichia coli | 100 | P03011 |
| blaTEM-1 | 100368-101228 | Beta-lactamase | Escherichia coli | 100 | AAR06285 |