TABLE 2.
Quantitation of tetrapeptide in DTZ-treated cells relative to the controla
| Expt | % Increase in tetrapeptide after DTZ exposure for:
|
|
|---|---|---|
| 3 h | 8 h | |
| 1 | NDb | 29 |
| 2 | 4 | 37 |
| Avg | 33 ± 4 | |
Extracts were prepared from E. coli MG1655 with or without DTZ (50 μM) treatment as described in the text. Tetrapeptide was quantitated by HPLC-MS. The amount of tetrapeptide in control samples is defined as 100%; the increase in tetrapeptide relative to the control sample is shown. In experiment 1, cells were taken directly from the wells of a Bioscreen plate; in experiment 2, cells were grown in a flask.
ND, not determined.