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Flow cytometric analysis of rhodamine 6G uptake and efflux. Uptake of fluorochrome was quantified by incubating cells of C. tropicalis isolates 21234 (A) and 21232 (B) in YEPD broth for 30 min with 100 μM rhodamine 6G (black line). Then, efflux was evaluated by quantifying the residual fluorescence of the cells after removal of the free dye and an additional incubation of 15 min in YEPD broth (gray area).
