TABLE 2.
Results of immunohistochemistry, urease testing, and PCR-SL for mouse stomach samplesa
| Mouse sample | Immunohistochemistry result
|
Urease test result (gastric tissue) | PCR-SL test result (gastric tissue) | ||
|---|---|---|---|---|---|
| Antrum | A-B | Body | |||
| Negative control 1 | − | − | − | − | − |
| Negative control 2 | − | − | − | − | − |
| Negative control 3 | − | − | − | − | − |
| Negative control 4 | − | − | ND | − | − |
| Negative control 5 | − | − | − | − | − |
| Samples for isolate 1 | |||||
| Positive control 1 | ++ | ++ | ++ | + | + |
| Positive control 2 | ND | ND | ++ | + | + |
| Positive control 3 | ND | ND | ++ | + | + |
| Positive control 5 | ++ | ++ | ++ | + | + |
| OA 1 | − | − | − | − | − |
| OA 2 | − | − | − | − | − |
| OA 3 | ND | ND | − | − | − |
| OA 4 | − | − | − | − | − |
| OA 6 | − | − | − | − | − |
| OA 7 | ND | ND | − | − | − |
| OA 9 | ND | ND | − | − | − |
| OA 10 | − | − | − | − | − |
| Samples for isolate 2 | |||||
| Positive control 1 | ++ | ++ | ++ | + | + |
| Positive control 2 | ++ | ++ | ++ | + | + |
| Positive control 3 | ++ | ND | ND | + | + |
| Positive control 4 | ND | ND | ++ | + | + |
| Positive control 5 | ++ | ++ | ++ | + | + |
| Positive control 6 | ++ | ++ | ++ | + | + |
| Positive control 7 | ++ | ND | ND | + | + |
| Positive control 8 | ++ | ND | ND | + | + |
| Positive control 9 | ++ | ND | ND | + | + |
| Positive control 10 | ++ | ++ | ++ | + | + |
| OA 1 | − | − | + | + | + |
| OA 2 | − | − | − | − | − |
| OA 3 | ND | − | − | − | − |
| OA 4 | + | ND | ND | + | + |
| OA 5 | + | ND | + | + | + |
| OA 6 | − | − | − | − | + |
| OA 7 | ND | ND | + | + | + |
| OA 8 | − | ND | ND | − | + |
| OA 9 | − | − | − | + | + |
| OA 10 | − | − | − | + | + |
a
−, negative result; + positive result; ++, strong positive result; A-B, antrum-body transition; ND, not determined, i.e., specific stomach region was not present; OA, omeprazole and amoxicillin treatment. Positive control animal 4, OA animal 5, and OA animal 8 from the isolate 1 group died during the experiment.