Skip to main content
. 2005 Nov;79(22):14189–14196. doi: 10.1128/JVI.79.22.14189-14196.2005

TABLE 3.

Ebola virus protein sequences recognized by murine CD8+ T cellsa

EBOV protein Epitope sequence Amino acid position Restriction Result of indicated assay
Survivale
IFN-γ ICCb IFN-γ ELISPOTc 51Crd
GP VSTGTGPGAGDFAFHK 141-155 H-2d 0.24/0.02 ND 47 10/10
WIPYFGPAAEGIYTE 531-545 H-2b 0.4/0.08 715 3 0/50
NP VYQVNNLEEIC 44-52 H-2b 1.06/0.11 410 73 10/10
GQFLSFASL 148-156 H-2b 0.88/0.11 295 45 19/20
SFKAALSSL 279-287 H-2b 0.63/0.04 ND 43 17/18
DAVLYYHMM 663-671 H-2b 0.99/0.11 910 57 17/20
VP24 KFINKLDALH 159-168 H-2d 0.52/0.09 535 46 20/20
NYNGLLSSI 171-179 H-2d 0.38/0.09 135 51 20/20
PGPAKFSLL 214-222 H-2d 3.34/0.09 957 43 18/20
VP30 KFSKSQLSLLCETHLR 181-196 H-2d 0.12/0.3 ND 55 18/20
181-196 H-2b 0.45/0.15 190 47 19/20
DLQSLIMFITAFLNI 231-245 H-2b 0.7/0.15 165 32 15/20
231-245 H-2d 0.42/0.17 ND 38 16/20
VP35 CDIENNPGL 45-53 H-2b 1.99/0.15 40 80 19/20
MVAKYDHL 138-145 H-2b 1.63/0.15 249 79 20/20
TVPQSVREAFNNL 190-202 H-2d 0.62/0.23 ND 43 18/20
RNIMYDHL 225-323 H-2b 1.53/0.15 202 87 19/20
PGFGTAFHQLVQVICK 233-248 H-2d 0.63/0.23 ND 47 17/20
VP40 LRIGNQAFLQEFVLPP 150-165 H-2b 0.7/0.05 101 53 19/20
AFLQEFVLPPVQLPQ 160-175 H-2d 0.45/0.22 ND 47 17/20
YFTFDLTALK 171-180 H-2d 0.41/0.22 ND 38 16/20
TESPEKIQAI 232-141 H-2d 0.6/0.22 ND 37 18/20
Lassa N RPLSAGVYMGNLSSQ H-2b 1.5/0.14 425 0/20
H-2d 0.7/0.18 325 0/10
MLR H-2b 0/40
H-2d 0/20
PBS H-2b 0/20
H-2d 0/20
a

Mice were vaccinated with 2.0 × 106 IU of VRP expressing Ebola virus GP, NP, VP24, VP30, VP35, or VP40. On day 28, mice were given a booster, and 7 days later, splenocytes were collected. In some cases (GP, VP35, VP30, and VP40 in BALB/c mice [H-2d]) mice received multiple booster vaccinations or larger doses of vaccine before sequences containing potential epitopes were identified.

b

Splenocytes were used ex vivo for identification of peptides that induced IFN-γ-expressing CD8+ T cells. Data shown represent intracellular IFN-γ data after 5 h of restimulation with peptide. The data are expressed as follows: percentage of peptide-induced IFN-γ-positive/percentage of background IFN-γ-positive cells.

c

Data are expressed as numbers of spot-forming cells per 106 peripheral blood mononuclear cells in the IFN-γ ELISPOT assay. Representative data are shown for one experiment. ND, not detected in assay.

d

Data are expressed as percent specific lysis in a chromium release assay with haplotype-matched, peptide-pulsed targets at a 25:1 effector:target ratio, except for the GP107 peptide, which is the specific lysis at a 33:1 ratio.

e

Data are expressed as numbers of mice surviving challenge/number tested. Peptide-specific restimulated lymphocytes were adoptively transferred to filovirus-naïve mice approximately 4 h before mice were challenged with 1,000 PFU of mouse-adapted Ebola virus. Control mice received PBS, or 5 × 106 cells from a mixed-lymphocyte reaction culture or 5 × 106 Lassa N-specific CTLs. No clinical symptoms were observed in any surviving mice.