FIG. 4.

Role of anti-VP4 antibodies in the plasma-dependent enhancement of CVB-induced IFN-α production by PBMC. CVB3 (a) or CVB4 (CVB4E2) (b) was preincubated for 1 h at 37°C with either plasma at optimal dilution obtained from 5 healthy subjects, eluted anti-VP4 antibody fractions at various concentrations obtained from the plasma specimen using VP4-coated plates, anti-VP4-depleted plasma, or control antibodies eluted from mock-infected plates as described in Materials and Methods.