TABLE 2.

Detection by ELISA of anti-VP4 antibodies in plasma from healthy subjects and patients with IDDM

Subject group (n)	Anti-VP4CVB3		Anti-VP4CVB4
	No. (%) with positive detectiona	Index valueb	No. (%) with positive detectiona	Index valueb
Healthy subjects 40	14 (35)	0.9 ± 0.32	6 (15)	0.72 ± 0.34
IDDM patients 40	25 (62.5)c	1.88 ± 1.09d	32 (80)e	1.96 ± 0.85d

^aNumber of individuals with positive antibody detection by ELISA.

^bIndex values of anti-VP4 antibodies detected by ELISA. Microtiter plates were coated with VP4 dissociated from either CVB3 or CVB4 (CVB4E2). In the same way, microtiter plates were coated with proteins obtained from a mock dissociation procedure. The immunoassay cutoff value was determined by adding the specimen absorbance on mock infection plates to the blank absorbance on VP4 plates. The index value is obtained by calculating the specimen/cutoff value ratio. Data are presented as means ± standard deviations.

^cP < 0.02 versus healthy subjects (χ² test).

^dP < 0.001 versus healthy subjects (Student's t test).

^eP < 0.001 versus healthy subjects (χ² test).