FIG. 1.

Phosphorylation variants of VP22 as GFP-VP22 fusion proteins. (A) Line drawing of the GFP-VP22 (G22) open reading frame showing GFP (black box) and the 301 amino acids of the VP22 WT (white box). The serine (S)-to-alanine (A) or S-to-glutamic acid (E) substitutions present in the plasmids expressing the GFP-VP22P− (G22P−) or GFP-VP22P+ (G22P+) mutant are shown. These substitutions were introduced by PCR mutagenesis. (B) Live transfected Cos-1 cells expressing either the G22, G22P−, or G22P+ proteins, examined by confocal microscopy 24 h posttransfection.