FIG. 2.

Characterization of VP22 expressed in G22, G22P− or G22P+ virus infections. (A) Monolayers of Vero cells were infected with G22v, G22P−v, or G22P+v at a multiplicity of infection of 10, and total cell lysates were harvested after 10 h. Equal amounts of lysate were analyzed by SDS-PAGE followed by Western blotting with an antibody against VP22. (B) Vero cells were infected at a multiplicity of infection of 10 with the WT viral s17, GFP-VP22 expressing G22v, or the recombinant viruses G22P−v and G22P+v and incubated in a labeling medium containing [³²P]orthophosphate. Protein cell lysates were immunoprecipitated using an antibody directed against VP22 and then analyzed by Western blotting using an antibody directed against GFP. (C) SDS-PAGE analysis of the immunoprecipitated proteins and autoradiography. The positions of VP22 (➤) and GFP-VP22 (*) are shown.