FIG. 8.

HSV-1 expressing the P+ version of VP22 displays a growth defect in MDBK cells. (A) Monolayers of MDBK cells were infected with WT, Δ22 G22P−, or G22P+ viruses at a multiplicity of infection of 0.02. At various times after infection, total virus was harvested by combining the cells and medium and the samples were titrated on Vero cells. (B) Monolayers of MDBK cells were infected with Δ22, G22, G22P−, or G22P+ viruses at a multiplicity of infection of 10. At various times after infection, total cell extracts were made from infected cells and analyzed by Western blotting for VP22 and ICP0.