FIG. 4.
Cytoplasmic accumulation of late BALF4 mRNA is EB2 independent. (A) RNA from 293BMLF1-ko cells transiently transfected with a control vector or expression plasmids encoding proteins as indicated at the top of the figure were submitted to RT-PCR analysis using specific primers to detect the BALF4 mRNA. The PCR (25 cycles) was done with various amounts of RT product and in the presence of [α-32P]dCTP. The PCR products were loaded on a 6% polyacrylamide gel and visualized by autoradiography. (B) Expression of the BALF4 protein (gp110) was monitored by immunofluorescence. The 293BMLF1-ko cells were transiently transfected with expression plasmids encoding proteins as indicated on the left of the figure. After the cells were labeled with the anti-BALF4 antibody, they were observed on a UV fluorescence microscope. The BALF4 protein stained red.