TABLE 1.
List of primers used
| Gene and primer | Sequence (5′ → 3′) | B95-8 relative position |
|---|---|---|
| U6 snRNA | ||
| Forward | CGCTTCGGCAGCACATATAC | |
| Reverse | AAAATATGGAACGCTTCACGA | |
| β-Actin | ||
| Forward | GCTGCGTGTGGCTCCCGAGGAG | |
| Reverse | ATCTTCATTGTGCTGGGTGCCAG | |
| BALF4 | ||
| Forward | GGAGTCGTAGGCAAATTGGA | 157922-157941 |
| Reverse | TCAAGAACCTGACGGAGCTT | 158125-158145 |
| BBRF1 | ||
| Forward | AAGATCTGCCAGCTCCTGAA | 115017-115036 |
| Reverse | ATGAACTCCTCCACCACGTC | 115197-115216 |
| BcLF1 | ||
| Forward | GACAATTATCAAAAAACCACC | 133313-133332 |
| Reverse | GGTGCAGTTTGTAGTG | 133528-133543 |
| BDLF1 | ||
| Forward | CAGATTTGAAAGTGGTAGTGTC | 133283-133301 |
| Reverse | TTATCTTAACCAGCAAGTGGCCG | 132400-132422 |
| BdRF1/BVRF2 | ||
| Forward | CACTATCAGGTAACGCAGGAG | 148710-148728 |
| Reverse | TCAAGCCAGCGTTTATTCAGC | 149723-149744 |
| BFRF3 | ||
| Forward | GGGAGGCTCAAAGAAGTTACCTG | 61628-61650 |
| Reverse | ATGAAGAAACAGAGGGGGTCGC | 61882-61903 |
| BFRF3 (for cloning) | ||
| Forward | GCCCAGATCTCGAGCAGCACGCCGGCTGCCC | 61510-61524 |
| Reverse | GCCCTCTAGACTACTGTTTCTTACGTGCCCCGCG | 62014-62037 |
| BLLF1 | ||
| Forwarda | TGTGCTGATAGAGGCTGGTG | 90018-90037 |
| Reverseb | TGACACCAAGTCCATCTCCA | 90672-90691 |
| BMRF1 | ||
| Forward | CCAGACATACGGTCAGTCCATCTC | 80881-80904 |
| Reverse | TGCTTCACTTTCTTGGGGTGC | 81071-81091 |
| BORF1 | ||
| Forward | GCCCCTCGAGCAAGGTCCAGGGGTCCGTCG | 75242-75259 |
| Reverse | GCCCTCTAGAGAATCACCTCCCAGTCAGAG | 76308-76333 |
| BVRF1 | ||
| Forward | GGGCAACCTGAACTTTACCA | 145991-146010 |
| Reverse | CGGCGATGATTTTCTCTAGG | 146219-146238 |
a
Primer 299.
b
Primer 300.