FIG. 3.

PDTC has no major effect on IRES dependent translation. At 6 h p.i. the level of IRES-dependent protein synthesis in HeLa cells, infected with HRV2 (multiplicity of infection, 100), was determined by metabolic labeling for 1 h; 125 μM PDTC or 125 μM cycloheximide (CHX) was added 15 min before the labeling and present during the period of the pulse. The amount of incorporated [³⁵S]methionine/cysteine was determined by protein precipitation and measurement by scintillation counting. Error bars indicate the standard deviation from experiments carried out in triplicate.