FIG. 5.

PDTC decreases the replication of positive and negative strand RNA of HRV2. HeLa cells were infected with HRV2 (multiplicity of infection, 20). At the start of viral RNA replication at 4 h p.i. PDTC was added where indicated (+). Total RNA was isolated 1, 2, 4, 6, 8, and 13 h p.i. The level of positive- and negative-strand HRV2 RNA was quantitated by Northern blot analysis using strand-specific probes generated by primer extension in the presence of [γ-³²P]dCTP and visualized by autoradiography. Analysis of the 28S rRNA ensured an equal loading.