FIG. 1.

Induction of hNIC expression and MHC-I downregulation. (A) Induction of hNIC expression. TRExBJAB-cDNA5, TRExBJAB-hNIC, TRExBCBL1-cDNA5, and TRExBCBL1-hNIC cells were stimulated with or without 1 μg/ml doxy for 0, 24, 48 or 72 h. Whole-cell lysates were used for immunoblotting with anti-Flag (αNIC) and antitubulin (αTubulin) antibodies. (B) Downregulation of MHC-I surface expression. TRExBCBL1-cDNA5 (black lines), TRExBCBL1-hNIC (red lines), and TRExBCBL1-RTA (blue lines) cells were treated with 1 μg/ml doxy for 0, 24, and 48 h, fixed, and then reacted with the indicated antibodies for flow cytometry.