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. 2005 Nov;79(22):14371–14382. doi: 10.1128/JVI.79.22.14371-14382.2005

FIG. 6.

FIG. 6.

Role of RBP-Jκ in the hNIC-mediated activation of vIL-6 promoter activity. (Top) hNIC-mediated activation of vIL-6 requires RBP-Jκ expression. RBP-Jκ−/− OT11 and RBP-Jκ+/+ OT13 MEFs were transfected with vIL-6 promoter luciferase reporters and pGK-β-gal together with pcDNA5 vector or pDNA5-hNIC. The mutant vIL-6 promoter luciferase reporters used were pvIL-6(−507), pvIL-6(−472), pvIL-6(−412), and pvIL-6(-m412) (only the last part of the plasmid designation is shown in the figure). At 48 h posttransfection, cell lysates were used for reporter assays. Luciferase activity is presented as the average of three independent experiments. (Bottom) Inhibition of the hNIC-induced activation of vIL-6 promoter activity by RBP-Jκ dominant-negative (DN) mutant. Wild-type (pGL3) or mutant vIL-6 promoter luciferase reporter together with pGK-β-gal was transfected into 293T cells with pDNA5-hNIC alone or with pcDNA5-hNIC and pcDNA5-RBP-Jκ DN vectors. At 48 h posttransfection, cell lysates were used for reporter assays. Luciferase activity is presented as the average of three independent experiments.