FIG. 7.

Z-KO LCLs are more sensitive than WT LCLs to Fas-induced apoptosis in vitro. (a) Early-passage Z-KO, R-KO, or WT LCLs from two different donors were subjected to treatment with anti-Fas antibody for 48 h. Following treatment, cell viability was assessed as described in Materials and Methods. Data shown represent the mean of a representative experiment done in triplicate; error bars indicate standard deviation. (b) WT LCLs, Z-KO LCLs carrying a control vector (Z-KO-vector), and Z-KO LCLs carrying a BZLF1 expression vector (Z-KO-ZpZ) were subjected to anti-Fas treatment, followed by an assessment of viability. Data shown represent the mean of a representative experiment done in duplicate; error bars indicate standard deviation.