FIG. 2.

Semiquantitative analysis of IL-11 mRNA expression in C3H mice infected with C. trachomatis MoPn. The PCR products from murine IL-11 (413 bp) primers after 28 cycles of amplification (lanes 1 to 5) and from GAPDH (536 bp) primers after 18 cycles (lanes 6 to 10) were analyzed on a 2% agarose gel (A) and after Southern blotting with an internal probe (B). Then, 10-μl portions of sample were loaded onto the gels as follows: uninfected control mice (lanes 1 and 6), MoPn-infected mice at 48 hpi (lanes 2 and 7) and at 72 hpi (lanes 3 and 8), a negative control of amplification with no cDNA added to the reaction mix (lanes 4 and 9), and cDNA from uninfected McCoy cells used as positive control for the RT and PCRs (lanes 5 and 10).