TABLE 2.

Analysis of cytokine and NO release by dTHP-1 cells cultivated alone or in coculture with C. trachomatis-infected polarized HeLa cells

Supernatanta	Concn (pg/ml) ± SD
	TNF-α	IL-1β	IL-6	IL-8	NO
U sup	-	-	11 ± 1.5	12.2 ± 4.4	ND
E sup	-	-	11.7 ± 3.2	33.1 ± 15.8*	ND
L2 sup	-	-	14.5 ± 9.5	47.4 ± 18.2*	ND
U-cocult. sup	35.6 ± 3.9	113.1 ± 48	71 ± 49	978 ± 322	-
E-cocult sup	41.5 ± 6.4	114.0 ± 33	85.8 ± 48.7	1,010 ± 439	-
L2-cocult sup	38.3 ± 5.3	112.3 ± 62.2	82.7 ± 28.7	1,037 ± 426	-
dTHP-1/RPMI sup	46.6 ± 5.1	60.2 ± 26.2	0.45 ± 0.2	ND	14.9

^aU, E, L2 sup, U-, E-, and L2-cocult sup represent basal supernatants from HeLa cells uninfected or infected with C. trachomatis E and L2 cultivated alone or in coculture with dTHP-1 cells, respectively, and collected at 48 hpi. Supernatant from dTHP-1 cells incubated for 48 h in the presence of RPMI only was used as a control for the spontaneous production of cytokines and NO as measured by ELISA and the Griess method, respectively. The cytokine concentrations presented here represent the average values from three independent experiments. Significant differences in cytokine concentrations present in infected HeLa cell supernatants (E sup, L2 sup) or in infected coculture supernatants (E-cocult sup, L2-cocult sup) compared to the respective negative controls (U sup and U-cocult sup, respectively) are indicated by an asterisk (P < 0.05). -, not detected; ND, not done.