FIG. 1.

Creation of KSHV-GFP1 recombinant virus. (A) Strategy for generation of KSHV-GFP1 recombinant virus. The structure of the first 30 kb of the KSHV genome is shown at the top. Viral genes are indicated as open boxes. The selective marker GFP-puro under the control of the CMV IE promoter was integrated between the ORF 11 and ORF K2 genes in the KSHV genome by homologous recombination in BCBL-1 cells with the recombination plasmid pKSGFP. Genomic structure of the resulting reconstituted virus KSHV-GFP1 is shown below. (B) Southern analysis of virion DNA isolated from induced BCBL-1 and KSHV-Vero supernatants. The probes used were the full-length vIL-6 gene (left panel), or the enhanced green fluorescence protein gene (right panel). (C) Photomicrograph of Vero cells infected with KSHV-GFP1 and selected with puromycin. (Left column) Phase. (Right column) Fluorescence for GFP.