FIG. 4.

(A) Stable expression of VP3/M3 reduces IBDV-induced cytopathic effects. Monolayers of BSC1 and BSC1/M3H were either mock infected or infected with IBDV at a multiplicity of infection of 0.1 PFU/cell. Phase-contrast images at a magnification of ×200 were recorded at 72 h p.i. (B) Effects of IBDV infection on the subcellular distribution of the M3 polypeptide. Mock- and IBDV-infected BSC1/M3H cells were processed for confocal laser scanning microscopy using specific antisera. Green (Alexa 488) corresponds to VP2, and red (Alexa 594) corresponds to the His tag of the M3 polypeptide. The blue signal (ToPro-3) corresponds to nuclear staining. The merge panels show the overlays of the three fluorescent signals.