FIG. 1.

Schematic representation of BMV cDNA constructs used to clone in the T-DNA region of the binary vector pCB301. The names of the constructs are in bold, and the molecule(s) produced upon transient expression is listed to the right of the constructs. Only the restriction enzyme cleavage sites used to make the constructs are shown. Three constructs used to express the three BMV RNAs are shown first. The boxes labeled with LB and RB denote the left border and right border of the T-DNA sequence, respectively. Large arrows labeled 35S denote the double CaMV 35S promoter elements. The long rectangles represent the protein coding sequences, and the names of the proteins are within the rectangles. The cloverleaf structure represents the 3′ tRNA-like structure of BMV RNAs. The curved arrows represent the cis-cleaving ribozyme sequence. WT, wild type. Construct pCB-302 used to express the BMV proteins from conventional mRNAs is shown at the bottom. All of the transgenes are cloned between the NcoI and XbaI restriction sites.