FIG. 2.

BMV RNA replication and infection in Tobacco initiated by agroinfiltration. (A) Ethidium bromide-stained agarose gel showing that BMV RNAs launched from T-DNA plasmids replicate to high levels. In this and all other panels, the type of sample from which the material was extracted is indicated above the gel image. The identities of BMV RNA2 and RNA3 are indicated to the right of the gel image. The other bands are RNAs from tobacco. (B) Comparison of minus- and plus-strand BMV RNA accumulation in agroinfiltrated tobacco and transfected barley protoplasts. The sources of the cells used are above the gel image, while the identities of the RNA bands are shown to the right of the gel image. Barley protoplasts (protop.) were harvested at 24 and 48 h after transfection, while the agroinfiltrated plants were collected at 1 to 5 days after infiltration. (C) SDS-PAGE demonstrating that BMV capsid protein is produced in Agrobacterium-infiltrated leaves and in systemic leaves. The infiltrated leaves were harvested 5 days after infiltration, while the systemic leaves were harvested 8 days after infiltration. Two independently infiltrated leaves were examined, while the lane labeled “BMV” shows a preparation of BMV CP purified from infected barley. Lane M, molecular mass standards. (D) Detection of BMV virions by transmission electron microscopy in samples harvested from the agroinfiltrated and noninoculated leaves. The white bar represents 50 nm.