FIG. 5.

Northern blot analyses of the effects of BMV proteins on BMV RNA accumulation. (A) Effects of the 1a and 2a proteins produced independently of BMV RNAs. The plasmids agroinfiltrated into particular tobacco leaves are noted above the gel image. The symbols + and − denote the presence and absence of a plasmid in the Agrobacterium strain used to infiltrate plants, respectively. Every sample was tested in two independently infiltrated leaves and processed for RNA analysis. We did not coexpress a protein with the RNA from which it was derived, since this combination resulted in silencing of the RNA (data not shown), in agreement with the results of Iyer and Hall (38). Also, these results were reproducible in at least three independent experiments that contained different combinations of the expression plasmids. The asterisk denotes what we presume to be a degradation product that comes from RNA1. This RNA is not present in the absence of agroinfiltration. (B) Effects of the movement and coat proteins on BMV RNA accumulation. The symbols used are as explained above. dpi, days postinfiltration. The asterisk denotes what we presume to be a degradation product that comes from RNA1. This RNA is not present in the absence of agroinfiltration.